recombinant mouse csf2 (R&D Systems)
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recombinant mouse csf2
Recombinant Mouse Csf2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+csf2/Recombinant+Mouse+GM-CSF+Protein/bio_rxiv__64898__2026__03__22__713539-316-49-53
Average 96 stars, based on 429 article reviews
Recombinant Mouse Csf2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+csf2/Recombinant+Mouse+GM-CSF+Protein/bio_rxiv__64898__2026__03__22__713539-316-49-53
Average 96 stars, based on 429 article reviews
recombinant mouse csf2 - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "Depletion and replacement of tissue resident macrophages in mice with germ-line deletion of a conserved enhancer in the Csf1r locus"
Article Title: Depletion and replacement of tissue resident macrophages in mice with germ-line deletion of a conserved enhancer in the Csf1r locus
Journal: bioRxiv
doi: 10.64898/2026.03.22.713539
Figure Legend Snippet: (A) Bone marrow-derived macrophages (BMM) from WT and Fireko mice were generated by culture in CSF2 for 7 days followed by staining and flow cytometry analysis. F4/80/CD11b + cells were gated based on MHCII expression. The relative proportions of MHCII High and Low subsets and their CSF1R MFI are shown. Data show mean and standard deviation, 2-way ANOVA with Sidak’s multiple comparison test. (B) CSF2-derived BMM were incubated with pH Rodo E.coli bioparticles (BP) for 1 h followed by surface staining and analysis by flow cytometry for BP uptake (%) and median fluorescence intensity (MFI) in cell subsets. Data show mean and standard deviation. (C) Representative images of bone marrow from WT or Fireko mice cultured in CSF1, CSF2 or both for 3 days prior to the addition of RANKL and continued culture until day 7. Cells were stained for tartrate-resistant acid phosphatase (TRAP, purple)
Techniques Used: Derivative Assay, Generated, Staining, Flow Cytometry, Expressing, Standard Deviation, Comparison, Incubation, Fluorescence, Cell Culture
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Recombinant:Article Title: Obesity-Activated Lung Stomal Cells Promote Myeloid-Lineage Cell Accumulation and Breast Cancer Metastasis Article Snippet: Bone marrow cells that invaded into the conditioned media were quantified using a hemocytometer, then cytospun onto slides, fixed in methanol and stained using antibodies for CD45 (ThermoFisher Scientific; 14-0451-82), CD11b (Novus Biologicals; NB110-89474), Ly6G (Abcam; ab25377). .. Invasion was also quantified in response to serum-free DMEM supplemented with 10 ng/mL of Article Title: Homozygous kinase-dead Csf1r mutation in outbred mice reveals essential and redundant functions of tissue resident macrophages Article Snippet: .. 10 7 cells were seeded on 100 mm square bacteriological plastic (Sterilin, Thermo-Fisher, Australia) in 25 ml of complete medium (RPMI + 10% FBS, 25 U/mL penicillin, and 25 μg/mL streptomycin (Gibco, Thermo-Fisher, Australia) and differentiated for 7 days with the addition of recombinant CSF1-Fc (100 ng/ml) or Article Title: Depletion and replacement of tissue resident macrophages in mice with germ-line deletion of a conserved enhancer in the Csf1r locus Article Snippet: .. 10 7 BM cells were seeded on 100 mm square bacteriological plastic (Sterilin, Thermo-Fisher, Australia) in 25 ml of complete medium (RPMI + 10% FBS, 25 U/mL penicillin, and 25 μg/mL streptomycin (Gibco, Thermo-Fisher, Australia) and differentiated for 7 days with the addition of recombinant CSF1-Fc (100 ng/ml) or Article Title: Obesity-Activated Lung Stromal Cells Promote Myeloid Lineage Cell Accumulation and Breast Cancer Metastasis Article Snippet: Bone marrow cells that invaded into the conditioned media were quantified using a hemocytometer, then cytospun onto slides, fixed in methanol and stained using antibodies for CD45 (ThermoFisher Scientific; 14-0451-82, Waltham, MA, USA), CD11b (Novus Biologicals; NB110-89474, Centennial, CO, USA), and Ly6G (Abcam; ab25377, Cambridge, MA, USA). .. Invasion was also quantified in response to serum-free DMEM supplemented with 10 ng/mL of Article Title: Colony-Stimulating Factor 1 Receptor Blockade Inhibits Tumor Growth by Altering the Polarization of Tumor-Associated Macrophages in Hepatocellular Carcinoma. Article Snippet: After addition of three volumes of NH4Cl solution (0.8% NH4Cl solution; Beyotime Institute of Biotechnology, Jiangsu, China), the mixture was incubated on ice for 10 min to remove red blood cells; the cells were then spun down and resuspended in cold PBS with 2% FBS (38). .. The harvested cells were cultured in DMEM containing 10% FBS and supplemented with 10 ng/ml recombinant mouse CSF1 (R&D Systems) or 10 ng/ml other:Article Title: Discovery of BPR1R024, an Orally Active and Selective CSF1R Inhibitor that Exhibits Antitumor and Immunomodulatory Activity in a Murine Colon Tumor Model. Article Snippet: Aliquots of the cell suspension were cultured in DMEM medium containing 10% FBS supplemented with either 10 ng/mL Cell Culture:Article Title: Colony-Stimulating Factor 1 Receptor Blockade Inhibits Tumor Growth by Altering the Polarization of Tumor-Associated Macrophages in Hepatocellular Carcinoma. Article Snippet: After addition of three volumes of NH4Cl solution (0.8% NH4Cl solution; Beyotime Institute of Biotechnology, Jiangsu, China), the mixture was incubated on ice for 10 min to remove red blood cells; the cells were then spun down and resuspended in cold PBS with 2% FBS (38). .. The harvested cells were cultured in DMEM containing 10% FBS and supplemented with 10 ng/ml recombinant mouse CSF1 (R&D Systems) or 10 ng/ml |